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Jacob Cook
Jacob Cook

Breeding Log 1.04

We can't wait to embark upon this exciting journey with everyone - we couldn't have done it without you, and we are so grateful for your love and support. Thanks again for 10 years of breeding, feeding, and singing with the Monsters! Happy Monstering!

Breeding Log 1.04


There's lots more to discover in this landmark update to My Singing Monsters, and of course, it wouldn't be Anniversary Month without a few extra tricks up our sleeves... stay tuned! Thanks for 10 years of breeding, feeding, and singing with the Monsters! Happy Monstering!

That's not all - a huge collection of creepy Costumes are now available to buy or through breeding during the Spooktacle Seasonal Event! Some of these haven't been seen for many years, so we're excited to dust them off and put them on display!

The ancient magic of the wishing torches has been rediscovered! When you light a wishing torch, it improves the chances of breeding rare monsters! The more torches you have on an island, the better your chances! If you don't have the means to light a torch yourself, you can put out a call to your friends to ask them to light it for you - for free!

Main results: We included three trials involving 1671 participants; oxytocin was the only uterotonic drug that was used. The dose and route of administration of oxytocin varied among the included studies. Administration of oxytocin before and after the expulsion of placenta does not significantly influence the incidence of postpartum haemorrhage (blood loss greater than 500 ml) (risk ratio (RR) 0.81, 95% confidence interval (CI) 0.62 to 1.04; n = 1667, three trials); retained placenta (RR 1.54, 95% CI 0.76 to 3.11; n = 1667, three trials); length of third stage of labour (minutes) (mean difference (MD) -0.30, 95% CI -0.95 to 0.36; n = 1667, three trials); postpartum blood loss (ml) (MD 22.32, 95% CI -58.21 to 102.86; n = 181, two trials); changes in haemoglobin (g/dL) (MD 0.06, 95% CI -0.60 to 0.72; n = 51, one trial); blood transfusion (RR 0.79, 95% CI 0.23 to 2.73; n = 1667, three trials); the use of additional uterotonics (RR 1.10, 95% CI 0.80 to 1.52; n = 1667, three trials); the incidence of maternal hypotension (RR 2.48, 95% CI 0.23 to 26.70; n = 130, one trial) and the incidence of severe postpartum haemorrhage (blood loss 1000 ml or more) (RR 0.98, 95% CI 0.48 to 1.98; n = 130, one trial). No data on other maternal or neonatal outcome measures were available.

Winx is a bay mare bred in Australia by John Camilleri's Fairway Thoroughbreds. She was foaled on 14 September 2011 at Coolmore Stud, Jerrys Plains in the Hunter Region of New South Wales.[7] She was sired by the Irish-bred Street Cry, who won the Dubai World Cup in 2002 [8] before becoming a very successful breeding stallion in North America and Australasia. His other progeny included Zenyatta, Street Sense, Shocking and Whobegotyou.[9] Winx's dam, Vegas Showgirl, was a stakes winner in New Zealand who was bought by Fairway Thoroughbreds in 2008 as a broodmare prospect for A$455,000.[10]

Winx was retired to broodmare duty for the 2019 breeding season, which in Australia begins in September.[96] On 24 June 2019, her connections announced that her first mating would be with I Am Invincible.[97] On October 13, 2020, it was announced that Winx had lost her foal (a filly) being stillborn.[98]

After missing a breeding season the following year to give her time to recover, it was announced in late 2021 that Winx was in foal to the stallion Pierro.[99] She foaled a filly on the evening of 7 October.[100]

In our previous study, we observed a significantly higher daily H2S production in Lohmann laying hens than in Hy-line Gray laying hens (daily H2S production per kg average daily feed intake was 7.75 and 4.17 mg, respectively) [29]. However, whether this difference in H2S emissions was due to host miRNA regulation of the gut microbiota remained unelucidated. Therefore, in the present study, we determined the expression profiles of host-derived miRNAs in the cecal contents of these two breeds of laying hens to identify miRNAs showing significantly different expression and then predicted the target relationships between differentially expressed miRNAs and microbial genes related to H2S production. Finally, the effects of selected targeted miRNAs on H2S production in laying hens were verified through in vitro experiments. The results of the present study should provide a better understanding of the interkingdom regulatory relationships among miRNAs, cecal microbiota and H2S production in laying hens, providing a reference for the breeding of environmentally friendly laying hens. At the same time, miRNAs, which have been proven to regulate the production of H2S in the cecum of laying hens, could be used as a safe and clean additive to promote reductions in H2S emissions in the future.

The methionine synthetase genes (metH) Odosp_3416 (expressed by the bacterium Odoribacter splanchnicus) and BF9343_2953 (expressed by the bacterium Bacteroides fragilis NCTC 9343) were predicted to be targeted by gga-miR-222a (Table 1). The abundances of Odoribacter splanchnicus and Bacteroides fragilis NCTC 9343 and the levels of Odosp_3416 and BF9343_2953 expression were quantified to assess the effect of gga-miR-222a on bacterial abundance and gene expression (Fig. 5). The addition of gga-miR-222a had no effect on the abundances of the two bacteria in the intestinal content broth of the two breeds when comparing the blank groups (Fig. 5A). In contrast, the expression of the genes Odosp_3416 and BF9343_2953 in the LT (Odosp_3416, 3.648 0.72; BF9343_2953, 2.513 0.14) and HT (Odosp_3416, 5.837 1.04; BF9343_2953, 3.918 0.42) groups was significantly higher than that observed in the LB (Odosp_3416, 1 0; BF9343_2953, 1 0) and HB (Odosp_3416, 3.353 0.59; BF9343_2953, 2.199 0.64) groups (P metH (Odosp_3416 and BF9343_2953) expression in the intestinal content broths of the two breeds. However, the results also showed that gga-miR-222a did not significantly affect the abundances of target gene-related bacteria.

In summary, the results of the present study led to the first identification of host-derived miRNAs in the cecum of laying hens, and the expression profiles of miRNAs were shown to be different between different breeds. It was also demonstrated for the first time that the methionine synthetase genes (metH) Odosp_3416 (expressed by the bacterium Odoribacter splanchnicus) and BF9343_2953 (expressed by the bacterium Bacteroides fragilis NCTC 9343) could be targeted by gga-miR-222a and affected the production of H2S in laying hens. Additionally, gga-miR-222a could enter Bacteroides fragilis NCTC 9343, which increased its abundance in the logarithmic period. Therefore, different profiles of host-derived miRNAs in different breeds of laying hens could affect the production of H2S through gene expression regulation in H2S production-related bacteria. The regulation of H2S production in the cecum of laying hens by host miRNAs such as gga-miR-222a provides the possibility that if these miRNAs could be incorporated into the breeding of laying hens, they could promote the selection of low odor yield and environmentally friendly laying hen breeds.

Tomato varieties resistant to TYLCV could obviate or reduce the necessity of pesticide applications and/or plant seclusion (greenhouses or shadehouses) and could provide a stable and sustainable management alternative (Polston, 2003). Progress in breeding for TYLCV resistance was initially slow, due in part to the complex genetics of resistance and the presence of interspecific barriers between the wild and domesticated tomato species (Brunetti et al., 1997). Furthermore, until recently, most of the improved genetically resistant material has come from Israel. It is important for southern Florida to have a breeding and variety testing program under local environmental conditions and directed at existing market requirements. TYLCV-R varieties and germplasm adapted to the hot and humid Florida environment are now being developed by BHN Seed (Immokalee, FL), Harris Moran Seed Co. (Modesto, CA), Seminis Vegetable Seeds (Oxnard, CA), Syngenta Co. (Golden Valley, MN), University of Florida Tomato Breeding Program (Balm, FL), Sakata Seeds (Morgan Hill, CA), Hazera Seeds (Coconut Creek, FL), and others.

Evaluations of TYLCV-R varieties and germplasm have been conducted in Florida (Cushman and Stansly, 2006; Gilreath et al., 2000; Scott, 2004); however, this study provides information needed to revise current recommendations including new varieties and breeding lines previously not evaluated under low and high TYLCV pressure. Relative to susceptible varieties, such as Florida 47, Sebring (round), and Mariana (Roma-type), TYLCV-R varieties evaluated produced comparable yields under low virus pressure and greater yields under high virus pressure. However, resistant varieties have yet to be widely grown in Florida, probably due to a perception of lower fruit quality compared with traditional varieties such as Florida 47 and Sebring. Additionally, TYLCV-R varieties should also have resistance to other common diseases such as Fusarium crown rot (Fusarium oxysporum f.sp. radicis-lycopersici) and bacterial leaf spot [BLS (Xanthomonas vesicatoria, Xanthomonas euvesicatoria, Xanthomonas perforans, and Xanthomonas gardneri)]. The goal of this study was to compare the yield and quality of round and Roma-type tomato varieties/advanced breeding lines with resistance/tolerance to TYLCV to the current industry standards.

A visual assessment was administrated by 28 participants during a field day at first harvest 7 Apr. 2008 (Spring 2008 season) to evaluate earliness, plant vigor, fruit size, firmness, fruit quality (color and shape), potential yield, and overall plant rating using a 1-to-5 scale (1 = very poor, 5 = very good). The visual assessment was not administrated in Spring 2007 due to high TYLCV incidence. TYLCV-R varieties were coded and the names were not known to the participants making the ratings. The participants in the visual assessment had experience evaluating tomato varieties/advanced breeding lines, including tomato growers, breeders, tomato seed sales representative, extension agents, etc. 041b061a72


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